解剖学报

›› 2011, Vol. 42 ›› Issue (6): 825-831.doi: 10.3969/j.issn.0529-1356.2011.06.022

• 组织学胚胎学发育生物学 • 上一篇    下一篇

糖尿病对大鼠股骨头生长板分化和成骨的影响

陈海英1*; 陈小萍1; 郑建盛1; 陈少强2; 刘文革3; 唐军民4   

  1. 1.福建莆田学院医学院基础医学部，福建 莆田 351100; 2.福建医科大学人体解剖学与组织胚胎学系，福州 350003;3.福建医科大学附属协和医院骨科，福州 350003; 4.北京大学医学部人体解剖学与组织学胚胎学系，北京 100191
  • 收稿日期:2011-08-26 修回日期:2011-09-09 出版日期:2011-12-06
  • 通讯作者: 陈海英

Effect of diabetes on the differentiation and osteogenesis mineralization offemoral head growth plate in rats

  1. 1.Department of Basic Medicine, Medical College of Putian University, Fujian Putian 351100, China; 2.Department of Anatomy and Histology and Embryology, Fujian Medical University, Fuzhou 350004,China;3.Department of Orthopedics, Union Hospital of Fujian Medical University, Fuzhou 350001, China;4.Department of Anatomy and Histology and Embryology, Peking University Health Science Center, Beijing 100191, China
  • Received:2011-08-26 Revised:2011-09-09 Online:2011-12-06
  • Contact: CHEN Hai-ying

关键词: 糖尿病, 生长板, 胶原纤维, 蛋白聚糖, 血管内皮生长因子, 墨汁灌注, 原位杂交, 大鼠

Abstract: Objective To study the effect of diabetes on development of long bones.Methods The diabetic model(STZ) of the rat was established.Rats were randomly divided into 3 normal groups：5-week(CON1), 10-week(CON2) and 15-week(CON3) and 3 diabetic groups：5-week(DM1), 10-week(DM2) and 15-week(DM3),10 rats each group. The change of collagen fiber depositing was observed by Van Gieson staining method. The microvascular density was measured by the ink infusion method.The techniques of in situ hybridization and RT-PCR were used in this study in order to analyze the intensity of vascular endothelial growth factor (VEGF)mRNA expression and content of decorin,respectively.Results The thickness and cellular density of the cell pillar in the growth plate were obviously lower in the 15-week diabetic group than those in the control group(EM>P/EM> <0.05).The content of collagen in the calcified zone of the growth plate was higher than that in the control group(EM>P/EM> <0.05),and the content of decorin decreased in the 15-week diabetic group(EM>P/EM> <0.01),the intensity of VEGFmRNA expression increased(EM>P/EM> <0.01),the microvascular density increased.Conclusion The cellular proliferation,differentiation and its osteogenesis mineralization in the growth plate of the femoral head in the 15-week diabetic rats a

Key words: Diabetes, Growth plate, Collagen, Decorin, Vascular endothelial growth factor, Infusion with ink, EM>In situ/EM> Hybridization, Rat

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